Thermo Fisher Scientific Histone H2A.Z Recombinant Rabbit Monoclonal Antibody (RM215)
Histone H2A.Z 단백질을 인식하는 recombinant rabbit monoclonal 항체로, 높은 특이성과 재현성을 제공합니다. WB, ICC/IF, ELISA, Luminex 등 다양한 응용에 사용 가능하며, lot 간 일관성과 동물 유래 성분이 없는 포뮬레이션을 특징으로 합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| ELISA | 0.1–1 µg/mL |
| Luminex (LUM) | 0.1–1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM215 |
| Immunogen | Peptide corresponding to the C-terminus of human Histone H2A.Z (UniProt ID: P0C0S5-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2–7.4, with 50% glycerol, 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2661887 |
Product Specific Information
This antibody reacts to Histone H2A.Z protein, independent of post-translational modifications, and shows no cross-reactivity with other histone proteins.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Specific antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal clones for production.
Advantages of recombinant rabbit monoclonal antibodies:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to the rabbit immune repertoire
Target Information
Histones are basic nuclear proteins that form the nucleosome structure of eukaryotic chromatin. Each nucleosome consists of an octamer of core histones (H2A, H2B, H3, and H4) around which approximately 146 bp of DNA is wrapped. The linker histone H1 binds to linker DNA between nucleosomes, aiding in chromatin compaction. The Histone H2A.Z gene encodes a member of the histone H2A family and produces two transcripts through different termination motifs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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