Thermo Fisher Scientific CD27 Monoclonal Antibody (O323), APC, eBioscience
인간 CD27을 인식하는 O323 단클론 항체로, APC 형광 표지되어 유세포분석(Flow Cytometry)에 최적화됨. T세포 활성화 및 B세포 상호작용 연구에 유용. 651/660 nm에서 여기/방출, 4°C 암소 보관. 연구용으로만 사용.
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Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 5 µL (0.25 µg)/test
- Publications: View 53 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Cynomolgus monkey, Human, Mouse, Non-human primate, Rhesus monkey |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | O323 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10669588 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- APC-eFluor 780
- Biotin
- Brilliant UV 395, 563, 615, 737, 805
- Brilliant Violet 421, 480, 605, 711
- eFluor 450
- FITC
- NovaFluor Blue, Red, UV, Violet, Yellow series
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Request custom conjugation
Product Specific Information
Description:
The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and virtually all mature T cells, and is upregulated upon T-cell stimulation. CD27 binds to CD70, playing an important role in T cell–B cell interaction.
Applications Reported:
The O323 antibody has been reported for use in flow cytometric analysis.
Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Use 5 µL (0.25 µg) per test (final volume 100 µL). Cell number may range from 10⁵–10⁸ cells/test.
Fluorescence Characteristics:
- Excitation: 633–647 nm
- Emission: 660 nm
- Laser: Red Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD27 is a 50 kDa member of the TNF receptor superfamily, including CD40 and CD30, involved in regulating cell proliferation and death. Expression of CD27 and its ligand CD70 is primarily confined to lymphocytes. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells, and a subset of B cells. Upon T cell activation, CD27 expression increases, and soluble CD27 (sCD27) is released, serving as a prognostic marker in B cell malignancies. The CD27-binding protein SIVA is a pro-apoptotic molecule implicated in CD27-mediated apoptosis. Disorders linked to CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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