Thermo Fisher Scientific CD27 Monoclonal Antibody (LG.7F9), APC, eBioscience

Thermo Fisher Scientific CD27 Monoclonal Antibody (LG.7F9), APC, eBioscience™

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.06 µg/test
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Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 700
• APC-eFluor 780
• Biotin
• Brilliant UV 661
• Brilliant Violet 650
• Brilliant Violet 786
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

The LG.7F9 monoclonal antibody reacts with mouse CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells. In mouse, CD27 has been found on nearly all thymocytes excluding a population of CD46-CD8- precursors. CD27 binds to CD70 and plays an important role in T cell–B cell interactions and effector cell maturation. LG.7F9 cross-reacts with human and rat CD27.

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse splenocytes (≤ 0.06 µg/test, 100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test. Antibody titration is recommended for optimal performance.

Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD27 is a 50 kDa member of the TNF receptor superfamily, including CD40 and CD30. It regulates cell proliferation and death and is mainly expressed on lymphocytes. CD27 and CD70 interaction influences T-cell activation and B-cell signaling. Soluble CD27 (sCD27) serves as a prognostic marker for B-cell malignancies. Dysfunction of CD27 is associated with Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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