Thermo Fisher Scientific ErbB2 (HER-2) Recombinant Rabbit Monoclonal Antibody (SP3)

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 73 publications 73 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

View 10 publications 10 publications

Immunocytochemistry (ICC/IF)

-

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

proprietary

Class

Recombinant Monoclonal

Type

Antibody

Clone

SP3

Immunogen

Recombinant protein encoding extracellular domain of human c-erbB2

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.122 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10984335

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is breast carcinoma.

Target Information

ErbB2 (HER2) is a receptor tyrosine kinase that is overexpressed in some breast tumors. Herceptin, used in treatment of metastatic Her2-positive cancer, is monoclonal antibody targeting this kinase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.