Thermo Fisher Scientific CD4 Monoclonal Antibody (SP35)

Applications

Product Specifications

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using an EDTA buffer for 10 minutes followed by cooling at room temperature for 20 minutes. Following antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
A suggested positive control is tonsil or T-cell lymphoma.

Target Information

The CD4 antigen is involved in the recognition of MHC class II molecules and acts as a co-receptor for HIV. CD4 is primarily expressed in T helper cells but can also be found in monocytes, macrophages, and dendritic cells.
At the tissue level, CD4 expression is observed in thymus, lymph nodes, tonsils, spleen, and certain regions of the brain and gut.
CD4 contributes to T-cell activation through association with the T-cell receptor complex and protein tyrosine kinase Lck. It may also mediate neuronal damage in infectious and immune-mediated CNS diseases.
Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.