Thermo Fisher Scientific CD4 Monoclonal Antibody (OX35), Super Bright 600, eBioscience
Rat CD4 인식용 OX35 클론 단일클론 항체로, Super Bright 600 형광체 결합. Flow cytometry에 최적화되어 있으며 405 nm violet laser로 여기, 600 nm에서 방출. 4°C 암소 보관, 연구용으로만 사용.
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Thermo Fisher Scientific CD4 Monoclonal Antibody (OX35), Super Bright 600, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 1.0 µg/test | View 17 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rat |
| Published Species | Rat |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Super Bright™ 600 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OX35 |
| Conjugate | Super Bright™ 600 |
| Excitation / Emission Max | 414 / 601 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2762766 |
Available Formats
- Unconjugated
- APC
- FITC
- PE
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 780
- Custom conjugation available upon request
Product Specific Information
The OX35 monoclonal antibody reacts with rat CD4, a co-receptor involved in T cell receptor signaling through MHC class II on antigen-presenting cells. CD4 is expressed by CD4+CD8+ double-positive and CD4+ single-positive thymocytes, as well as CD4+ helper T cells in the periphery.
Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of rat splenocytes (≤ 1.0 µg/test)
Super Bright 600 is a tandem dye excited by a violet laser (405 nm) and emits at 600 nm. Recommended detection using a 610/20 bandpass filter.
Staining Buffer Recommendation:
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days at 4°C in the dark.
Excitation: 405 nm
Emission: 600 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD4 antigen is a co-receptor for MHC class II molecules and HIV. It is primarily expressed in T helper cells but can also be found in monocytes, macrophages, and dendritic cells. Tissue-level expression includes thymus, lymph nodes, tonsils, spleen, and certain non-lymphoid tissues. CD4 facilitates T-cell activation via association with Lck kinase and may mediate neuronal damage in immune-related CNS diseases. Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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