Thermo Fisher Scientific IL-13 Polyclonal Antibody, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
TPGPVRRSTS PPVALRELIE ELSNITQDQK TSLCNSSMVW SVDLTAGGFC AALESLTNIS SCNAIHRTQR ILNGLCNQKA SDVASSPPDT KIEVAQFISK LLNYSKQLFR YGH.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Rat IL-13. The anti-Rat IL-13-specific antibody was purified by affinity chromatography employing an immobilized Rat IL-13 matrix.

Sandwich ELISA:
To detect Rat IL-13 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Rat IL-13 (500-P224Bt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Rat IL-13.

Western Blot:
To detect Rat IL-13 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Rat IL-13 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Target Information

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and plays a role in allergen-induced asthma through mechanisms independent of IgE and eosinophils. It regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5, and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.