Thermo Fisher Scientific GM-CSF Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MAPTRSPNPV TRPWKHVDAI KEALSLLNDM RALENEKNED VDIISNEFSI QRPTCVQTRL KLYKQGLRGN LTKLNGALTM IASHYQTNCP PTPETDCEIE VTTFEDFIKN LKGFLFDIPF DCWKPVQK.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Rat GM-CSF. Anti-Rat GM-CSF-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Rat GM-CSF by sandwich ELISA (100 µL/well), use 0.25–1.0 µg/mL of this antibody. In conjunction with PeproTech Polyclonal Anti-Rat GM-CSF (500-P225) as a capture antibody, detection of at least 0.2–0.4 ng/well of Recombinant Rat GM-CSF is possible.

Western Blot:
For detection of Rat GM-CSF by Western Blot, use 0.1–0.2 mg/mL. In combination with compatible development reagents, detection limit is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Packaging:
500-P225BT-1MG is provided as 2 × 500 µg.

Target Information

The GM-CSF gene encodes a cytokine regulating the production, differentiation, and function of macrophages and granulocytes. The active protein form exists as a homodimer in the extracellular space. This gene is located in a cluster at chromosome region 5q31, associated with interstitial deletions in 5q- syndrome and acute myelogenous leukemia. Related genes include interleukins 4, 5, and 13. GM-CSF is involved in promoting tissue inflammation. Elevated cytokine levels, including GM-CSF, have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome. Mice lacking this gene or its receptor develop pulmonary alveolar proteinosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.