Thermo Fisher Scientific IL-22 Polyclonal Antibody, Biotin, PeproTech
Biotin으로 표지된 토끼 폴리클로날 항체로, 마우스 IL-22 단백질 검출에 사용됩니다. Western blot과 ELISA에 최적화되어 있으며, 항원 친화 크로마토그래피로 정제되었습니다. 고순도 재조합 IL-22를 면역원으로 사용하여 높은 특이성과 감도를 제공합니다.
- 카탈로그번호
- 500-P223BT-xxxx (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL-22 Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| ELISA | 0.25–1.0 µg/mL | 2 publications |
| In vitro Assay (IV) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Murine IL-22 |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929891 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein
MLPVNTRCKL EVSNFQQPYI VNRTFMLAKE ASLADNNTDV RLIGEKLFRG VSAKDQCYLM KQVLNFTLED VLLPQSDRFQ PYMQEVVPFL TKLSNQLSSC HISGDDQNIQ KNVRRLKETV KKLGESGEIK AIGELDLLFM SLRNACV
Preparation
Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-22. Anti-Murine IL-22-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA
To detect mIL-22 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine IL-22 (500-P223) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant mIL-22.
Western Blot
To detect mIL-22 by Western Blot analysis, use at 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant mIL-22 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Packaging
500-P223BT-1MG will be provided as 2 × 500 µg.
Target Information
IL-22, also known as IL-10-related T-cell derived inducible factor, is an alpha-helical cytokine belonging to the IFN–IL-10 family, which includes IL-19, IL-20, IL-24, IL-26, IL-28, IL-29, and type I/II interferons. It is produced mainly by activated T cells and NK cells.
In humans, the IL-22 gene is located on chromosome 12q and is structurally related to IL-10. IL-22 acts through a heterodimeric receptor complex composed of IL-22R1 and IL-10R2. Binding induces JAK-STAT signaling and regulates genes associated with innate immunity, particularly in nonimmune cells such as keratinocytes, hepatocytes, and epithelial cells of the skin, digestive, and respiratory systems.
IL-22 is co-expressed with IL-17A and IL-17F by Th17 cells and plays roles in inflammation, host defense, and tissue repair. It is upregulated during chronic inflammation and mediates STAT3 activation, promoting antimicrobial responses and wound healing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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