Thermo Fisher Scientific IL-22 Polyclonal Antibody, PeproTech
Mouse IL-22를 검출하기 위한 Rabbit Polyclonal Antibody로 Western blot, ELISA 등에 사용 가능. 항원 친화 크로마토그래피로 정제된 고순도 제품. Recombinant Murine IL-22를 면역원으로 제작되어 높은 특이성과 감도를 제공. 연구용으로만 사용.
- 카탈로그번호
- 500-P223-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL-22 Polyclonal Antibody, PeproTech
Thermo Fisher Scientific IL-22 Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | — |
| ELISA | 0.5–2.0 µg/mL | 2 publications |
| Blocking Assay (BLOCK) | — | 1 publication |
| In vitro Assay (IV) | — | 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Murine IL-22 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929887 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
MLPVNTRCKL EVSNFQQPYI VNRTFMLAKE ASLADNNTDV RLIGEKLFRG VSAKDQCYLM KQVLNFTLED VLLPQSDRFQ PYMQEVVPFL TKLSNQLSSC HISGDDQNIQ KNVRRLKETV KKLGESGEIK AIGELDLLFM SLRNACV.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-22. Anti-Murine IL-22-specific antibody was purified by affinity chromatography employing an immobilized Murine IL-22 matrix.
Sandwich ELISA:
To detect Murine IL-22 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. In conjunction with PeproTech Biotinylated Anti-Murine IL-22 (500-P223Bt) as a detection antibody, it enables detection of at least 0.2–0.4 ng/well of Recombinant Murine IL-22.
Western Blot:
To detect Murine IL-22 by Western Blot analysis, use at 0.1–0.2 µg/mL. With compatible secondary reagents, detection limit for Recombinant Murine IL-22 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Target Information
IL-22, also known as IL-10-related T-cell derived inducible factor, is an alpha-helical cytokine belonging to the IFN-IL-10 family (including IL-19, IL-20, IL-24, IL-26, IL-28, IL-29, and type I/II interferons).
Produced mainly by activated T cells and NK cells, IL-22 acts via the heterodimeric receptor complex IL-22R1 and IL-10R2. Binding induces JAK-STAT signaling. A soluble IL-22BP receptor antagonizes IL-22 binding and signaling.
IL-22 primarily targets nonimmune cells (skin, digestive, respiratory systems, hepatocytes, keratinocytes). It functions as an effector cytokine of Th17 cells, regulating genes associated with innate immunity, antimicrobial protein induction, and keratinocyte mobility. IL-22 is critical for host defense against extracellular pathogens and promotes wound healing. Upregulated in activated T cells, IL-22 mediates IL-23-induced acanthosis and dermal inflammation through STAT3 activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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