Thermo Fisher Scientific MKL1 Monoclonal Antibody (OTI2B9), TrueMAB
Human MKL1 단백질(aa 165–397)을 인식하는 Mouse IgG1 단클론 항체로, Western blot에 최적화되어 있습니다. 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도 유지. 연구용으로 세포핵 내 신호전달 및 근육세포 분화 관련 연구에 적합.
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Applications
- Western Blot (WB)
Tested Dilution: 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2B9 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 165–397 of human MKL1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | –20 °C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The MKL1 protein interacts with the transcription factor myocardin, a key regulator of smooth muscle cell differentiation.
It is predominantly nuclear and may help transduce signals from the cytoskeleton to the nucleus.
A translocation event involving this gene and RNA-binding motif protein-15 has been associated with acute megakaryocytic leukemia.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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