Thermo Fisher Scientific MKL1 Monoclonal Antibody (OTI4F6), TrueMAB
Human MKL1 단백질(aa 165–397)에 특이적인 Mouse IgG1 단클론 항체. Western blot에 적합하며, 동결건조 형태로 제공. PBS buffer(8% trehalose) 보존, -20°C 보관. 연구용으로 세포핵 신호 전달 및 근육세포 분화 연구에 활용 가능.
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Applications
- Western Blot (WB)
Tested Dilution: 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4F6 |
| Immunogen | Human recombinant protein fragment (aa 165–397 of human MKL1) produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The MKL1 protein interacts with the transcription factor myocardin, a key regulator of smooth muscle cell differentiation. It is predominantly nuclear and may help transduce signals from the cytoskeleton to the nucleus.
This gene is involved in a translocation event that fuses MKL1 with RNA-binding motif protein-15 (RBM15), associated with acute megakaryocytic leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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