Thermo Fisher Scientific NCOA2 Monoclonal Antibody (OTI7G5), TrueMAB

Applications

• Western Blot (WB): Tested dilution 1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

SRC2 (steroid receptor coactivator 2), also known as nuclear receptor coactivator 2 (NCOA2 or TIF2), assists nuclear hormone receptors in regulating gene expression related to cell growth, development, and homeostasis.
Members of the nuclear hormone receptor superfamily include steroid and class II nuclear receptors, characterized by:

1. N-terminal transcriptional activation domain
2. Central DNA-binding domain
3. C-terminal hormone-binding domain

Before hormone binding, steroid receptors remain inactive in complexes with heat-shock protein 90 and other stress proteins. Hormone binding induces conformational changes that activate receptors, allowing them to bind DNA enhancer elements and regulate transcription via coactivator proteins such as SRC2.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.