Thermo Fisher Scientific AmpFLSTR NGM SElect PCR Amplification Kit

Applied Biosystems™ AmpFLSTR™ NGM SElect™ PCR Amplification Kit

The NGM SElect Kit is a highly robust, single amplification Short Tandem Repeat (STR) multiplex kit developed for human identification laboratories wishing to utilize the expanded European Standard Set of Loci along with the highly polymorphic SE33 locus. The kit combines the latest advances in primer synthesis, buffer enhancement, and thermal cycling optimization, enabling high sensitivity and improved STR performance for forensic casework and database samples in one easy workflow.

Key Features

• Exceptional data quality and discrimination capabilities support international data sharing
• Enhanced performance on inhibited and degraded samples
• Greater sensitivity for low DNA input
• Maximized concordance with previously typed samples
• Optimized workflow and streamlined implementation

Data Quality Worth Sharing

Designed to deliver powerful discrimination and data quality, the NGM SElect Kit supports cross-border data sharing initiatives. It meets ENFSI and EDNAP requirements and includes the expanded European Standard Set of Loci (ESSL).

Loci Configuration

The kit simultaneously amplifies 17 loci:
D3S1358, vWA, D16S539, D2S1338, D8S1179, D21S11, D18S51, D19S433, TH01, FGA, Amelogenin, D10S1248, D22S1045, D2S441, D1S1656, D12S391, and SE33 (PET dye channel).
All amplicons are under 450 bp, maximizing performance across degraded forensic samples.

Powerful Discrimination

The combination of loci provides the highest discrimination power among AmpFLSTR kits, minimizing adventitious matches in large datasets. Applications include:

• Complex kinship analysis
• Disaster victim identification
• Missing person investigations

Enhanced Performance on Challenging Samples

Using advanced PCR technology, the kit enables full profile recovery from inhibited and degraded samples, even those containing high concentrations of inhibitors such as humic acid.

Increased Sensitivity

Provides superior allele recovery at low input levels. Standard 29-cycle protocol ensures balanced profiles; a validated 30-cycle protocol increases signal strength for lower DNA inputs.

Maximized Concordance

Maintains identical primer sequences for core STR loci shared with SGM Plus, Identifiler, and SEfiler Plus kits to ensure data compatibility and reduce reanalysis needs. SE33 locus primers are optimized for concordance with SEfiler Plus.

Confident Data Interpretation

Improved primer synthesis yields cleaner baselines and reduced dye artifacts, enhancing interpretation accuracy for low-level samples. Compatible with GeneMapper ID-X software for streamlined analysis.

Easy Implementation

Single-kit solution for both database and casework samples. Enzyme included in master mix simplifies setup. Shortened cycling time (~1 hour faster) increases throughput. Available in:

• 200 reactions kit
• 1000 reactions kit (for automated platforms)

Note: For Forensic or Paternity Use Only.

Important Notice

Purchase grants a non-transferable license for forensic or paternity testing only. Other uses such as chimerism analysis, cancer research, or teaching are excluded.
For licensing details, visit thermofisher.com/HIDlicensing.

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