Thermo Fisher Scientific NA-XTD Influenza Neuraminidase Assay Kit

Invitrogen™ NA-XTD™ Influenza Neuraminidase Assay Kit

The NA-XTD™ Influenza Neuraminidase Assay Kit is a next-generation chemiluminescence-based assay that provides a longer signal read-out compared to the first-generation NA-Star® Influenza Neuraminidase Inhibitor Resistance Detection Kit.
This kit includes detection reagents and microplates, eliminating the need for luminometers equipped with reagent injectors and improving ease-of-use.
It also provides complete assay protocols for quantitating sensitivity to neuraminidase inhibitors in various influenza virus isolates including human influenza types A and B, A/H1N1 pandemic, avian, equine, and porcine viruses.

Key Product Features

• Superior performance: Improved, long-lived light emission kinetics and read-time flexibility
• High sensitivity: High assay signal-to-noise, detection of low-virus concentrations, and wide assay dynamic range
• Simple instrumentation requirement: No reagent injectors or luminometer needed
• Multiple applications: Neuraminidase inhibitor IC50 assays and cell-based virus quantitation

Long-Lived Light Emission Kinetics & Read-Time Flexibility

The NA-XTD™ assay yields much longer-lived light emission kinetics than the NA-Star® assay, eliminating the need for luminometer instrumentation with reagent injectors and enabling read-time flexibility and batch-mode processing of assay plates.
NA-XTD™ assay signal half-life is approximately 2 hours, longer than the 5–10 minute half-life of the NA-Star® assay signal.
IC50 values determined from data collected immediately or up to 3 hours following addition of NA-XTD™ Accelerator solution are identical.

High Sensitivity

The NA-XTD™ chemiluminescent assay provides higher detection sensitivity, better low-end detection limit, higher assay signal-to-noise ratio, and wider assay dynamic range than fluorescent assays with the MUNANA substrate.
It offers 2–50-fold higher sensitivity by signal-to-noise ratio than MUNANA-based fluorescence assays, depending on the virus isolate.
The assay provides a dynamic detection range of 3–4 orders of magnitude of neuraminidase enzyme concentration, compared to 2–3 orders with fluorescent MUNANA assays.
This wide range enables determination of IC50 values over various virus concentrations, eliminating the need for virus titration prior to IC50 assays.

Simple Instrumentation Requirement

The NA-XTD™ assay can be used for virus quantitation in media samples from 96-well microplates or other virus cultures for monitoring viral growth or infection, or for performing viral inhibition assays in a cell-based system.
A small sample of culture media can be directly assayed with NA-XTD™ reagents, permitting multiple samples to be assayed over time.

Multiple Applications in One Complete Kit

The kit includes:

• NA-XTD™ Assay Buffer for virus sample and inhibitor dilution
• NA Sample Prep Buffer with optional Triton® X-100 for enhanced NA activity
• NA-XTD™ Accelerator Solution for high-intensity light emission
• NA-Star™ Detection Microplates (96-well solid white plates) for optimum performance

A comprehensive assay protocol is included, covering virus and inhibitor dilution recommendations, plate layout for NI sensitivity assays, virus quantitation, and literature references.
Compatible with a wide range of luminometers, including single-mode and multi-mode instruments, with no need for injectors.

For Research Use Only. Not for use in diagnostic procedures.

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