Thermo Fisher Scientific PMEL Polyclonal Antibody
PMEL 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체로, Western blot 및 IHC(P) 분석에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 인간 시료에 반응합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encoding aa 1–150 of human gp100 (PMEL, UniProt ID: P40967-1, NCBI Node ID: 9606) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.123 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2549958 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is melanoma tissue.
Target Information
Melanoma is a malignant tumor of melanocytes, which are found predominantly in the skin but also in the bowel and the eye (uveal melanoma).
Although it is one of the rarer types of skin cancer, it causes the majority of skin cancer–related deaths.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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