Thermo Fisher Scientific Ku80 Polyclonal Antibody
Rabbit polyclonal antibody targeting human Ku80 protein. Validated for WB, IHC(P), and ICC/IF applications. Recommended antigen retrieval with EDTA buffer. Suitable for DNA repair and recombination research. For research use only.
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- PA532472
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- pk
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Item | Description |
|---|---|
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from the C-terminus of human Ku80 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2549941 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 1 mM EDTA buffer (pH 8.0) for 10 minutes followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is placenta tissue.
Target Information
The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein, also known as ATP-dependent DNA helicase II or DNA repair protein XRCC5.
Ku is the DNA-binding component of DNA-dependent protein kinase and functions together with the DNA ligase IV–XRCC4 complex in repairing DNA double-strand breaks by non-homologous end joining and completing V(D)J recombination events.
This gene complements the Chinese hamster xrs-6 mutant, which is defective in DNA double-strand break repair and V(D)J recombination.
A rare microsatellite polymorphism in this gene is associated with cancer in patients with varying radiosensitivity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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