Thermo Fisher Scientific PIASy Polyclonal Antibody
PIASy 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 ICC/IF에 사용 가능. Human, Mouse, Rat에 반응하며, Protein G로 정제된 액상 형태. SUMO ligase 관련 연구 및 전사 조절 경로 분석에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 3–5 µg/mL |
| Immunocytochemistry (ICC/IF) | 1:10–1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptides corresponding to residues 44–60 and 187–198 of human PIASy |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2164379 |
Product Specific Information
These sequences are 95% and 100% homologous in mouse and rat.
Suggested positive control: liver, Jurkat.
Target Information
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT, p53/TP53, Wnt, and steroid hormone signaling pathways. Involved in gene silencing and mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4, and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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