Thermo Fisher Scientific Phospho-Chk2 (Thr26, Ser28) Polyclonal Antibody
Chk2 단백질의 Thr26 및 Ser28 인산화 상태를 특이적으로 인식하는 rabbit polyclonal 항체. Western blot, IHC, ICC/IF에 사용 가능. 인간, 마우스, 랫트 반응성. 1 mg/mL 농도의 액상 형태로 제공되며 -20°C에서 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human CHEK2 (Accession O96017), corresponding to amino acid residues around phosphorylated Ser28. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2817173 |
Product Specific Information
This antibody detects endogenous levels of Chk2 only when phosphorylated at Thr26 and Ser28.
Target Information
CHEK2 (CHK2) is a serine/threonine kinase involved in the DNA damage checkpoint pathway. Mutations in CHK2 are linked to various cancers. CHK2 is activated by DNA damage and phosphorylates key regulators of cell cycle control, including tumor suppressor proteins such as BRCA1. It functions as a putative tumor suppressor, with mutations associated with Li-Fraumeni syndrome and predisposition to sarcomas, breast cancer, and brain tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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