Thermo Fisher Scientific Kappa Light Chain Monoclonal Antibody (TB28-2), Biotin, eBioscience
인간 면역글로불린 카파 경쇄를 인식하는 단클론 항체로, Flow Cytometry에 최적화됨. Biotin 결합형으로 다양한 형광 형식 제공. 고순도 Affinity chromatography 정제, 0.5 mg/mL 농도, 4°C 암소 보관. 연구용으로만 사용 가능.
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Thermo Fisher Scientific Kappa Light Chain Monoclonal Antibody (TB28-2), Biotin, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.25 µg/test
Publications: -
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TB28-2 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1907419 |
| Filtration | 0.2 µm post-manufacturing filtered |
Additional Formats
Product Specific Information
The monoclonal antibody TB28-2 recognizes the kappa light chain of human immunoglobulin. The Ig kappa chain (~22 kDa) consists of one variable and one constant region. Two kappa chains together with heavy chains form the complete immunoglobulin structure. Expression of Ig kappa is typically found on more than half of B lymphocytes.
Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of normal peripheral blood cells. Recommended usage ≤ 0.25 µg/test (100 µL total volume). Cell number should be optimized empirically (10⁵–10⁸ cells/test). Titrate for best assay performance.
Target Information
Immunoglobulins consist of two heavy and two light chains (kappa or lambda, ~22.5 kDa). Kappa and lambda chains differ by antigenic properties of their constant regions. The kappa:lambda ratio is approximately 70:30. Antibodies to kappa light chain are useful for identifying leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas. Demonstration of clonality in lymphoid infiltrates indicates malignancy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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