Thermo Fisher Scientific ROBO-1 Polyclonal Antibody
Thermo Fisher Scientific의 ROBO-1 Polyclonal Antibody는 인간, 마우스, 랫, 개에서 반응성을 보이는 친화 정제 항체입니다. Western blot, IHC, ICC/IF, ELISA, IP 등 다양한 응용에 적합하며, ROBO-1 단백질 연구용으로 설계되었습니다. -20°C에서 안정적으로 보관 가능합니다.
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Thermo Fisher Scientific ROBO-1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| ELISA | 1:30,000–1:60,000 |
| Immunoprecipitation (IP) | 1:500–1:3,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to the C-terminal region near amino acids 1625–1650 of Human ROBO-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This affinity-purified antibody is directed against human ROBO-1 protein. A BLAST analysis suggests reactivity with human, mouse, rat, and dog ROBO-1 proteins based on 100% homology for the immunogen sequence. Cross-reactivity will occur with all isoforms of ROBO-1. Cross-reactivity with homologues from other species has not been determined.
Target Information
Specialized cells at the midline of the central nervous system (CNS) direct growth cone behavior. In vertebrates, insects, and C. elegans, midline cells produce guidance cues such as nectins (attractants) and slit (repellents). Robo is an axon guidance receptor belonging to the immunoglobulin superfamily, conserved from fruit flies to mammals. Robo acts as a receptor for Slit and functions cell-autonomously. Non-crossing axons express high levels of Robo, whereas crossing axons express low levels before reaching the midline and high levels after crossing.
Robo1 and Robo2 are human homologs of the Drosophila protein Roundabout. Robo1 is homologous to the C. elegans gene sax3, and Robo2 to the zebrafish gene astray.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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