Thermo Fisher Scientific SLIT2 Polyclonal Antibody
Human SLIT2 단백질에 특이적인 Rabbit Polyclonal Antibody로, WB, IHC, ELISA, IP에 사용 가능. Affinity chromatography로 정제된 액상 항체이며, -20°C 보관. 신경 발달 연구 및 SLIT2 단백질 발현 분석에 적합.
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Thermo Fisher Scientific SLIT2 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 2 µg/mL |
| ELISA | 1:5,000–1:50,000 |
| Immunoprecipitation (IP) | 1:500–1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chicken, Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to aa 475–500 of Human SLIT-2 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This affinity purified antibody is directed against human SLIT-2 protein. BLAST analysis suggests reactivity with human and chicken SLIT-2 based on 100% homology for the immunogen sequence.
Cross-reactivity is expected with SLIT-2 from Tetraodon nigroviridis (94% positive by BLAST).
The core 13 amino acids of the immunogen are also 100% homologous to SLIT-2 from mouse, rat, bovine, and Xenopus laevis.
Cross-reactivity with SLIT-2 homologues from other species has not been determined.
Target Information
SLIT2 proteins are ligands for Robo receptors and play a vital role in axonal migration and navigation during neural development.
Several cleavage products through alternate splicing are reported for SLIT2 proteins.
The C-terminal cleavage proteins are more diffusible, while the larger N-terminal protein is more tightly cell-associated.
SLIT2 is expressed in tissues such as fetal lung, kidney, and adult spinal cord.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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