Thermo Fisher Scientific Gli2 Polyclonal Antibody
Mouse Gli-2 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ELISA, IP 등 다양한 응용에 사용 가능. Affinity chromatography로 정제된 고순도 항체로 -20°C에서 장기 보관 가능. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 – 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–20 µg/mL |
| ELISA | 1:15,000 – 1:60,000 |
| Immunoprecipitation (IP) | 1:500 – 1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Affinity purified antibody prepared from whole rabbit serum immunized with a synthetic peptide corresponding to amino acids from an internal region of Mouse Gli-2. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.02 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 0.15 M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Directed against mouse Gli-2 protein. A BLAST analysis suggests cross-reactivity with Gli-2 from mouse and rat sources based on 100% sequence homology with the immunogen. Reactivity with Gli-2 from other species is not known.
Target Information
Gli-2 belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass function as transcription factors binding DNA through zinc finger motifs containing conserved H-C links. Gli family zinc finger proteins mediate Sonic hedgehog (Shh) signaling and act as potent oncogenes in embryonal carcinoma cells. The encoded protein localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression, playing a role in embryogenesis. Associated phenotypes include Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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