Thermo Fisher Scientific Granzyme B Monoclonal Antibody (16G6), Biotin, eBioscience
Mouse Granzyme B를 인식하는 Rat 모노클로날 항체로 Biotin으로 결합되어 있습니다. Flow cytometry, Western blot, IHC 등 다양한 응용에 적합합니다. 세포 내 염색 시 Foxp3 Staining Buffer Set 사용 권장. 4°C에서 암소 보관, 동결 금지.
- 카탈로그번호
- 13-8822-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Granzyme B Monoclonal Antibody (16G6), Biotin, eBioscience
Thermo Fisher Scientific Granzyme B Monoclonal Antibody (16G6), Biotin, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | - | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-Dependent | View 1 publication |
| Flow Cytometry (Flow) | Assay-Dependent | View publications |
Product Specifications
| Specification | Detail |
|---|---|
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 16G6 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_466954 |
| Filtration | 0.2 µm post-manufacturing filtered |
Product Specific Information
The 16G6 antibody reacts with mouse Granzyme B (GrB), a member of the granzyme serine protease family found in the granules of cytotoxic T cells and NK cells. Granzyme B (also known as CGL1 or CTLA-1) is crucial for inducing apoptosis in target cells through interaction with cytotoxic T cells. The mannose 6-phosphate receptor acts as a death receptor for Granzyme B during this process.
For intracellular staining and flow cytometric analysis using direct conjugates of anti-mouse Granzyme B, it is highly recommended to use the Foxp3 Staining Buffer Set (Product #00-5523). Other buffers may yield varying results.
Applications Reported:
This 16G6 antibody is reported for use in intracellular staining followed by flow cytometric analysis, western blotting, and immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections.
Applications Tested:
The biotinylated 16G6 antibody has been tested by immunoblotting (WB); a recommended starting concentration is 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
Granzyme B is a granzyme serine protease found in cytotoxic T cells and NK cells. It plays a key role in apoptosis induction by cleaving caspase-3 and initiating the caspase cascade. Granzyme B also triggers mitochondrial apoptosis via Bid protein cleavage. Granzymes (A–G) have been cloned from mouse CTLs and NK cells, while fewer are identified in humans.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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