Thermo Fisher Scientific Granzyme B Monoclonal Antibody (16G6), Biotin, eBioscience

Thermo Fisher Scientific Granzyme B Monoclonal Antibody (16G6), Biotin, eBioscience™

Applications and Tested Dilution

Product Specifications

Product Specific Information

The 16G6 antibody reacts with mouse Granzyme B (GrB), a member of the granzyme serine protease family found in the granules of cytotoxic T cells and NK cells. Granzyme B (also known as CGL1 or CTLA-1) is crucial for inducing apoptosis in target cells through interaction with cytotoxic T cells. The mannose 6-phosphate receptor acts as a death receptor for Granzyme B during this process.

For intracellular staining and flow cytometric analysis using direct conjugates of anti-mouse Granzyme B, it is highly recommended to use the Foxp3 Staining Buffer Set (Product #00-5523). Other buffers may yield varying results.

Applications Reported:
This 16G6 antibody is reported for use in intracellular staining followed by flow cytometric analysis, western blotting, and immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections.

Applications Tested:
The biotinylated 16G6 antibody has been tested by immunoblotting (WB); a recommended starting concentration is 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Target Information

Granzyme B is a granzyme serine protease found in cytotoxic T cells and NK cells. It plays a key role in apoptosis induction by cleaving caspase-3 and initiating the caspase cascade. Granzyme B also triggers mitochondrial apoptosis via Bid protein cleavage. Granzymes (A–G) have been cloned from mouse CTLs and NK cells, while fewer are identified in humans.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.