Thermo Fisher Scientific SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480)), Biotin, eBioscience

Applications and Tested Dilutions

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• eFluor 660
• PE
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

Description:
The eBioMC-480 (MC-480) antibody reacts with stage-specific embryonic antigen-1 (SSEA-1), a carbohydrate epitope expressed on early mouse embryos, murine embryonal carcinoma (EC) cells, embryonic stem (ES) cells, and murine/human germ cells (EG). No immunoreactivity is seen with undifferentiated human EC and ES cells. Differentiation of human EC increases SSEA-1 expression, while in mouse it decreases. SSEA-1 is also expressed on mature human granulocytes (CD15) and some monocytes, and is associated with cell adhesion, migration, and differentiation.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of the F9 cell line. Recommended ≤0.5 µg per test (in 100 µL final volume). Cell number should be determined empirically (10⁵–10⁸ cells/test). Antibody titration is recommended for optimal performance.

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

CD15 (Lewis X, Le(x); SSEA-1):
A trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on glycolipids, glycoproteins, and proteoglycans in granulocytes, mast cells, monocytes, macrophages, gastric mucosa, nervous system, and various tumor cells. Variants include sialyl-Lewis X and sulphated Lewis X. Cells with high Le(x) expression exhibit calcium-dependent self-aggregation, involved in embryo compaction and teratocarcinoma cell aggregation. Sialyl-Le(x) and sialyl-Le(a) act as selectin ligands. CD15 expression is used in the diagnosis of Hodgkin’s disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.