Thermo Fisher Scientific SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480)), Biotin, eBioscience
SSEA-1 항원을 인식하는 Biotin-conjugated mouse monoclonal antibody로, 인간 및 마우스 시료에 반응합니다. Flow cytometry에 최적화되어 있으며, 0.5 µg/test 권장. 0.5 mg/mL 농도의 액상 제품으로, 4°C 보관. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 4 publications |
| Immunocytochemistry (ICC/IF) | - | 8 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | 13 publications |
| Functional Assay | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioMC-480 (MC-480) |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_657625 |
Additional Formats
Product Specific Information
Description:
The eBioMC-480 (MC-480) antibody reacts with stage-specific embryonic antigen-1 (SSEA-1), a carbohydrate epitope expressed on early mouse embryos, murine embryonal carcinoma (EC) cells, embryonic stem (ES) cells, and murine/human germ cells (EG). No immunoreactivity is seen with undifferentiated human EC and ES cells. Differentiation of human EC increases SSEA-1 expression, while in mouse it decreases. SSEA-1 is also expressed on mature human granulocytes (CD15) and some monocytes, and is associated with cell adhesion, migration, and differentiation.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of the F9 cell line. Recommended ≤0.5 µg per test (in 100 µL final volume). Cell number should be determined empirically (10⁵–10⁸ cells/test). Antibody titration is recommended for optimal performance.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
CD15 (Lewis X, Le(x); SSEA-1):
A trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on glycolipids, glycoproteins, and proteoglycans in granulocytes, mast cells, monocytes, macrophages, gastric mucosa, nervous system, and various tumor cells. Variants include sialyl-Lewis X and sulphated Lewis X. Cells with high Le(x) expression exhibit calcium-dependent self-aggregation, involved in embryo compaction and teratocarcinoma cell aggregation. Sialyl-Le(x) and sialyl-Le(a) act as selectin ligands. CD15 expression is used in the diagnosis of Hodgkin’s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
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