Thermo Fisher Scientific SSEA3 Monoclonal Antibody (eBioMC-631 (MC-631)), Biotin, eBioscience
인간 및 마우스 반응성의 SSEA-3 단클론 항체로, Biotin 결합형입니다. 인간 배아 및 생식줄기세포의 표면 마커 검출에 사용되며, 유세포 분석에 최적화되어 있습니다. 고순도 친화 크로마토그래피 정제 및 0.2 µm 필터링 처리로 신뢰성 높은 연구 결과를 제공합니다.
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Thermo Fisher Scientific SSEA3 Monoclonal Antibody (eBioMC-631 (MC-631)), Biotin, eBioscience™
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.25 µg/test
View 2 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Rat / IgM |
| Recommended Isotype Control | Rat IgM Isotype Control (eBRM), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioMC-631 (MC-631) |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_657842 |
Additional Formats
Product Specific Information
Description:
The eBioMC-631 (MC-631) monoclonal antibody reacts with the carbohydrate moiety galactosylgloboside on Stage-specific embryonic antigen-3 (SSEA-3). Expression is restricted to the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), and human embryonic stem cells (ES), and is downregulated following differentiation.
Applications Reported:
This antibody has been reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of the 2102Ep cell line.
Recommended ≤ 0.25 µg per test (100 µL final volume).
Cell number should be empirically determined (10⁵–10⁸ cells/test).
Careful titration is recommended for optimal assay performance.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
SSEA-3 (Stage-specific embryonic antigen 3) is a cell surface antigen expressed along with SSEA-4, TRA-1-60, and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS).
In human cells, these markers are downregulated during differentiation, while SSEA-1 is absent in undifferentiated human stem cells and upregulated after retinoic acid-mediated differentiation.
In mouse stem cells, SSEA-1 expression decreases with differentiation, while SSEA-3 and SSEA-4 expression increase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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