Thermo Fisher Scientific CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), eBioscience
MIH5 클론의 PD-L1(B7-H1) 단클론 항체로, 마우스 및 랫트 반응성. Flow Cytometry, IHC 등 다양한 어플리케이션에 사용 가능. 0.5 mg/mL 농도의 액상 형태로 제공되며, 4°C에서 보관. Blocking 기능 및 기능적 분석용으로 적합.
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Thermo Fisher Scientific CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500 | 3 |
| Immunohistochemistry (IHC) | - | 17 |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 1 |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | 1 |
| Immunocytochemistry (ICC/IF) | - | 4 |
| Flow Cytometry (Flow) | 0.25 µg/test | 75 |
| ELISA | - | 1 |
| Neutralization (Neu) | Assay-Dependent | 12 |
| Functional Assay | Assay-Dependent | 3 |
| Inhibition Assays (IA) | - | 2 |
| Miscellaneous PubMed | - | 1 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, lambda |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MIH5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467780 |
Available Conjugated Formats
- Alexa Fluor 488
- Alexa Fluor 660
- APC
- Biotin
- Brilliant UV 615 / 661 / 737 / 805
- Brilliant Violet 421 / 650 / 786
- Functional Grade
- NovaFluor Red 700 / 755
- NovaFluor Yellow 690 / 700
- PE / PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 780
- Custom conjugation available upon request
Product Specific Information
The MIH5 monoclonal antibody reacts with mouse B7-H1 (PD-L1), a member of the B7 family (~40 kDa) and Ig superfamily. B7-H1 is expressed on leukocytes including T, B, NK, and DC cells, and acts as a ligand for PD-1. Interaction of PD-1 with PD-L1 or PD-L2 inhibits T and B cell responses. MIH5 is a blocking antibody.
Applications Reported:
Used for flow cytometric analysis, immunohistochemical staining (frozen sections), and blocking in functional assays.
For functional assays, use Functional Grade Purified MIH5 (Product #16-5982).
Fluorochrome-conjugated MIH5 is recommended for flow cytometry.
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Optimal use ≤0.25 µg/test (100 µL volume). Cell number: 10⁵–10⁸ cells/test. Titrate for optimal performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Programmed death receptor ligand 1 (PD-L1, B7-H1) binds to PD-1, negatively regulating TCR signaling, reducing proliferation and cytokine production. PD-L1 is expressed on many murine and human cancers and upregulated by IFN-γ, suggesting a role in tumor immune evasion.
For Research Use Only.
Not for diagnostic procedures or resale without authorization.
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