Thermo Fisher Scientific PAG1 Polyclonal Antibody
PAG1 단백질을 인식하는 Thermo Fisher Scientific의 rabbit polyclonal 항체로, WB, IHC, IP 등 다양한 실험에 사용 가능. 인간 및 마우스 반응성, Protein A 정제, PBS buffer에 15mM sodium azide 포함. 연구용으로만 사용.
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Applications
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant intracellular fragment (aa 97–432) of human Cbp (PAG) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2160060 |
Product Specific Information
This antibody recognizes the intracellular part of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains). It migrates on SDS-PAGE gels anomalously as an 80 kDa molecule.
Target Information
PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a 46 kDa transmembrane adaptor protein found in membrane rafts. Upon tyrosine phosphorylation by Src family kinases, PAG binds and activates Csk, a negative regulator of Src kinases. In B cells and mast cells, receptor signaling increases PAG phosphorylation and Csk binding; in T cells, receptor signaling causes dephosphorylation, loss of Csk binding, and increased activation of Lck.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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