Thermo Fisher Scientific Zap-70 Polyclonal Antibody
Rabbit polyclonal antibody targeting the C-terminal region of human ZAP-70. Validated for Western blot applications. Recommended for detecting ZAP70 in T-cells and NK cells. Supplied as a liquid, unconjugated form with 1 mg/mL concentration. Store at 4...
- 카탈로그번호
- PA119390
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Zap-70 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Bacterially expressed fusion protein representing the C-terminal 160 amino acids of human ZAP-70 with a histidine tag |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1088340 |
Product Specific Information
This antibody recognizes the C-terminal region of human ZAP70 protein tyrosine kinase (intracellular antigen).
ZAP70 is susceptible to degradation; therefore, it is recommended to use freshly prepared cell lysates with protease inhibitors to avoid non-specific staining of degradation products.
Western Blot: Reducing conditions are recommended.
Target Information
ZAP-70 is a cytosolic protein tyrosine kinase belonging to the Syk family. It is expressed exclusively in T-cells and natural killer (NK) cells and is essential for T-cell receptor (TCR) activation.
Upon TCR engagement, ZAP70 is phosphorylated on tyrosines 292, 315, and 319 in the interdomain B region between the SH2 and kinase domains.
- Phosphorylation of tyrosines 315 (Vav binding site) and 319 (Lck binding site) enhances ZAP70-mediated lymphocyte signaling.
- Phosphorylation of tyrosine 292 attenuates ZAP70 activation and signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.