Thermo Fisher Scientific CD103 (Integrin alpha E) Monoclonal Antibody (2E7), eBioscience
Mouse CD103 (Integrin alpha E) 인식하는 Armenian hamster 유래 단클론 항체로, Flow Cytometry, IHC, IP 등 다양한 응용 가능. PBS buffer에 0.5 mg/mL 농도로 제공되며, 4°C 보관. 높은 순도(>90%)와 낮은 응집도(<10%)로 안정적 실험 재현성 보장.
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Thermo Fisher Scientific CD103 (Integrin alpha E) Monoclonal Antibody (2E7), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 2 |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 1 |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | 2 |
| Immunocytochemistry (ICC/IF) | - | 2 |
| Flow Cytometry (Flow) | 1 µg/test | 58 |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| Functional Assay | Assay-Dependent | - |
| Inhibition Assay (IA) | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2E7 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467408 |
Additional Formats Available
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 563
- Brilliant UV 615
- Brilliant UV 737
- Brilliant Violet 421
- Brilliant Violet 480
- Brilliant Violet 711
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue 510
- PE
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 600
- Request custom conjugation available
Product Specific Information
Description:
The 2E7 monoclonal antibody reacts with mouse CD103 (Integrin alpha E). CD103 associates with integrin beta7 and is expressed on intraepithelial lymphocytes, a subset of peripheral lymphocytes, and dendritic epidermal T cells (DEC). E-cadherin binds to CD103, mediating lymphocyte homing to intestinal epithelium.
Applications Reported:
Used for flow cytometry, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. Also applicable in in vitro functional studies. For functional assays, use Functional Grade purified 2E7 (Product #16-1031).
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤1 µg/test (100 µL final volume). Cell count may range from 10⁵–10⁸ cells/test. Antibody titration recommended for optimal performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD103 (ITGAE, Integrin alpha E) is expressed on mucosa-associated T lymphocytes and activated TGF β-1 cells. It includes a 150 kDa alpha chain and a 120 kDa beta chain. CD103 is expressed by 0.5–2% of resting lymphocytes in peripheral blood and lymphoid organs. It stains a few bone marrow cells and rarely peripheral blood lymphocytes.
CD103 belongs to the integrin family, composed of alpha and beta chains. ITGAE encodes an I-domain-containing alpha integrin, which undergoes post-translational cleavage yielding disulfide-linked heavy and light chains. Together with beta7 integrin, CD103 forms the E-cadherin binding integrin known as human mucosal lymphocyte-1 antigen. It is preferentially expressed in intestinal intraepithelial lymphocytes (IEL) and may function in adhesion and activation.
Associated Diseases:
Hairy cell leukemia, splenic marginal zone lymphoma.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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