Thermo Fisher Scientific CD24 Monoclonal Antibody (M1/69), PE, eBioscience
Thermo Fisher CD24 Monoclonal Antibody (M1/69), PE 형은 마우스 CD24 항원을 검출하는 Rat IgG2b 항체로, Flow Cytometry 분석에 최적화되어 있습니다. 565/576 nm 형광 특성을 가지며, 0.03 µg/test로 사용 가능합니다. B세포 발달 단계 분석 및 면역세포 표지에 유용합니다.
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Thermo Fisher Scientific CD24 Monoclonal Antibody (M1/69), PE, eBioscience™
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.03 µg/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fish, Human, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M1/69 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465601 |
Available Formats
- Unconjugated
- Alexa Fluor 532
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 615 / 737 / 805
- Brilliant Violet 711 / 786
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue 510 / 610-30S
- NovaFluor Red 685 / 710
- NovaFluor Yellow 570 / 590 / 660
- PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5 / PerCP-eFluor 710
- Super Bright 436 / 600 / 645
- Request custom conjugation available
Product Specific Information
The M1/69 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen (HSA). This 35–50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and expressed by erythrocytes, thymocytes, peripheral lymphocytes, and myeloid lineage. CD24 is variably glycosylated, resulting in heterogeneity of molecular mass among different cell lineages. It is used to resolve stages of B lymphopoiesis in mouse bone marrow and interacts with P-selectin (CD62P).
Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of mouse splenocytes and thymocytes (≤0.03 µg/test).
A test is defined as the antibody amount staining a cell sample in 100 µL final volume. Recommended careful titration for optimal assay performance.
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, from pro-B cells to mature B cells. Plasma cell expression is low or negative. CD24 is also expressed on most B-lineage acute lymphoblastic leukemias, B-cell CCLs, and B-cell non-Hodgkin’s lymphomas. It may regulate B-cell proliferation, maturation, and autoimmunity control.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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