Thermo Fisher Scientific Phospho-SRC (Tyr419) Polyclonal Antibody, Alexa Fluor 488

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 4 publications 4 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:250

View 2 publications 2 publications

Product Specifications

Species Reactivity

Dog, Chicken, Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This antibody was produced against a chemically synthesized phosphopeptide derived from the region of Src that contains tyrosine 419. The antibody was affinity purified by sequential epitope-specific chromatography, then conjugated to Alexa FluorÒ 488 under optimal conditions. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SRC,uniProtId:P12931-1,ncbiNodeId:9606,antigenRange:419,antigenLength:536,antigenImageFileName:44-660A1_SRC_P12931-1_Rabbit.svg,antigenImageFileNamePDP:44-660A1_SRC_P12931-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

Dulbecco`s PBS, pH 7.3, with 0.2mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533713

Target Information

SRC is a cytoplasmic tyrosine kinase and proto-oncogene involved in the regulation of embryonic development and cell growth. SRC is involved in the regulation of growth and differentiation of eukaryotic cells. SRC associates with the cytoplasmic region of various cell surface receptors to facilitate signal transduction, and is also involved in cytoskeletal organization, cell cycle regulation, and apoptosis. In humans, the gene encoding SRC is present on chromosome 20. Activity of SRC is regulated by two tyrosine residues (Tyr 416 and Tyr527), and phosphorylation of these two sites have opposing effects. Phosphorylation of Tyr416 in the activation loop of the kinase domain upregulates enzyme activity while the phosphorylation of Tyr527 decreases enzyme activity. Mutations in the SRC gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same SRC protein have been found thus far.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.