Thermo Fisher Scientific Phospho-SRC (Tyr419) Polyclonal Antibody, Alexa Fluor 488
Alexa Fluor 488으로 표지된 SRC Tyr419 인산화 특이 폴리클로날 항체로, WB, IHC, ICC/IF에 사용 가능. Rabbit IgG 기반으로 다양한 종에 반응하며, 고순도 정제 및 안정적 형광 특성 제공. 연구용으로 세포 신호전달 및 SRC 활성 분석에 적합.
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Thermo Fisher Scientific Phospho-SRC (Tyr419) Polyclonal Antibody, Alexa Fluor 488
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 4 publications |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:250 | View 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Chicken, Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Chemically synthesized phosphopeptide derived from the region of Src containing tyrosine 419. Affinity purified by sequential epitope-specific chromatography, then conjugated to Alexa Fluor™ 488 under optimal conditions. |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | Dulbecco’s PBS, pH 7.3, with 0.2 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533713 |
Target Information
SRC is a cytoplasmic tyrosine kinase and proto-oncogene involved in the regulation of embryonic development and cell growth. It plays a key role in signal transduction, cytoskeletal organization, cell cycle regulation, and apoptosis. In humans, SRC is located on chromosome 20. Phosphorylation of Tyr416 activates SRC, while phosphorylation of Tyr527 inhibits its activity. Mutations in SRC may contribute to malignant progression of colon cancer. Two transcript variants encoding the same SRC protein have been identified.
Usage Notice
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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