Thermo Fisher Scientific Phospho-SRC (Tyr529) Polyclonal Antibody
SRC 단백질의 Tyr529 인산화 형태를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC/IF, IP 등 다양한 응용 가능. 항원 친화 크로마토그래피로 정제된 액상 형태. -20°C 보관, 연구용 전용.
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Thermo Fisher Scientific Phospho-SRC (Tyr529) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 11 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:500 | - |
| Immunocytochemistry (ICC/IF) | 1 µg/mL | - |
| Immunoprecipitation (IP) | - | View 3 publications |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Chicken, Human, Mouse, Rat |
| Published Species | Bovine, Dog, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Chemically synthesized phosphopeptide derived from human Src region containing tyrosine 529 (530 including initiating methionine). Sequence conserved in mouse, rat, chicken, and frog. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Dulbecco’s PBS, pH 7.3, with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533715 |
Target Information
SRC is a cytoplasmic tyrosine kinase and proto-oncogene involved in the regulation of embryonic development and cell growth. It associates with cytoplasmic regions of various cell surface receptors to facilitate signal transduction and is involved in cytoskeletal organization, cell cycle regulation, and apoptosis.
In humans, the SRC gene is located on chromosome 20. Activity is regulated by two tyrosine residues (Tyr416 and Tyr527), with phosphorylation of Tyr416 increasing activity and Tyr527 decreasing it. Mutations in SRC may contribute to malignant progression of colon cancer. Two transcript variants encoding the same SRC protein have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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