Thermo Fisher Scientific Phospho-Histone H2A.X (Ser139) Recombinant Rabbit Monoclonal Antibody (HisH2AXS139-1B3)
Recombinant rabbit monoclonal antibody specific for phospho-Histone H2A.X (Ser139). Suitable for WB, ICC/IF, and Flow Cytometry. Provides high specificity, sensitivity, and lot-to-lot consistency. Ideal for detecting DNA damage and phosphorylation even...
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Thermo Fisher Scientific Phospho-Histone H2A.X (Ser139) Recombinant Rabbit Monoclonal Antibody (HisH2AXS139-1B3)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1–1,000 ng/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | HisH2AXS139-1B3 |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Ser139 of human phospho histone H2A.X |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2896958 |
Additional Formats
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
Target Information
Histone H2A.X (H2AX) is a member of the histone H2A family, one of four core histones forming the nucleosome core particle. In eukaryotes, DNA double-strand breaks (DSBs) trigger phosphorylation of serine 139 at the carboxy terminus of H2AX, resulting in γ-H2AX.
Phosphorylation can be detected by Western blotting or immunofluorescence to assess DSB frequency. The phosphorylation process involves phosphatidylinositol 3-kinases, including ATM, DNA-PK, and ATR.
H2A.X consists of 143 amino acids and is synthesized in both G1 and S-phases. Its mRNA has polyA addition motifs and a polyA tail, contributing to replication-type histone mRNA stability. Two mRNA forms exist: one (~1600 bases, polyA+) and one (~575 bases, polyA–).
Histone H2A.X maps to the human chromosome region 11q23.2–q23.3 and plays a key role in DNA structure and repair, regulating MDC1 interaction at DNA damage sites.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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