Norgen Stool DNA Isolation Kit Dx

Norgen Stool DNA Isolation Kit Dx

SKU: Dx27600
For the rapid and simple purification of bacterial and host DNA from stool and fecal samples for in vitro diagnostic use.

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Features and Benefits

• CE-IVDR marked in accordance with European Commission Regulation (EU) No. 2017/746
• Ideal for use in in vitro diagnostic workflows
• Simultaneous isolation of both host DNA and microbial DNA (universal protocol)
• Eliminates PCR inhibitors including humic acids
• Fully compatible with Norgen’s Stool Nucleic Acid Collection and Transport Tubes
• High-quality DNA for sensitive downstream applications including PCR, qPCR, sequencing, and microarray

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This kit provides a convenient and rapid method to isolate total DNA from fresh, frozen, and preserved stool samples, including those preserved using Norgen’s Stool Nucleic Acid Collection and Preservation Devices Dx (Cat. Dx45660).
The universal protocol allows simultaneous isolation of total genomic DNA from microorganisms and host cells.
All traces of humic acids and other inhibitors are removed using Bead Tubes and a combination of chemical and physical homogenization and lysis, without phenol-chloroform extraction.
A simple spin column procedure yields high molecular weight DNA (up to 50 kb+), suitable for PCR, qPCR, NGS, and microarray applications.

NOTE: This product is not available for sale in the United States.

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Kit Specifications

Specification	Description
Maximum Stool Input	200 mg (fresh/frozen) or 400 μL (preserved)
Type of Stool Processed	Frozen, fresh, or preserved stool
Format	Spin Column
Maximum Column Binding Capacity	50 μg
Maximum Column Loading Volume	650 μL
Elution Volume	50 μL
Time to Complete 10 Purifications	30 minutes
Applications	PCR, qPCR, Southern Blot, Sequencing, Microarray Analysis

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Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature.
This kit is stable for 2 years after the date of shipment.

Component	Cat. Dx27600 (50 preps)
Lysis Solution	60 mL
Lysis Additive	6 mL
Binding Solution	7 mL
Wash Solution I	30 mL
Wash Solution II	22 mL
Elution Buffer	3 mL × 2
Bead Tube	50
Mini Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1

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Supporting Data

Figure 1. Higher Yields of DNA than Competitor Z

Stool DNA was isolated from 200 mg of fresh stool samples using Norgen’s Stool DNA Isolation Kit Dx and Competitor Z’s Kit.
10 µL of DNA from the elution was run on 1X TAE 1.2% agarose gel.
Norgen’s kit isolated much higher yields of DNA.
Marker = Norgen’s HighRanger DNA Ladder.

Norgen	Competitor Z

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Figure 2. Higher Quality DNA than Competitor Z

DNA quality measured by Nanodrop. Norgen’s Stool DNA Isolation Kit Dx isolated DNA of the highest quality.

Sample	A260/280 (OD)	A260/230 (OD)
Norgen	~2.0	~1.5
Competitor Z	~1.0	~0.3

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Figure 3. High Quality DNA confirmed by Real-time PCR

Stool DNA was isolated from 200 mg of fresh stool samples using Norgen’s kit and Competitor Z’s kit.
DNA quality confirmed by real-time PCR detecting 16S rRNA.
Earlier Ct values with Norgen’s samples indicate higher quality DNA.

Amplification Graph Parameters

• Y-axis: RFU (Relative Fluorescence Units) [0–2000]
• X-axis: Cycles [0–40]

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Figure 4. Taxonomic Classifications of 10 Fecal Microbiomes

A) Principal Coordinate Analysis (PCoA) showing taxonomic distribution up to class level.
B) Hierarchical clustering based on genus-level classifications.

Axis	Label
X-axis	Principal Coordinate 2
Y-axis	Principal Coordinate 1

Taxonomic Levels: Kingdom, Phylum, Class, Order, Family, Genus, Species

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Documentation

Protocols

• (Dx27600) Stool DNA Isolation Kit Dx - Protocol (50 Preps)

Application Notes

• Detection of Viral, Bacterial and Human Genomic DNA From Preserved Stool Samples
• Quantitative Adenovirus Detection Using Taqman Real-time PCR
• Comparative Study of DNA Isolated from Stool Using Norgen’s Kits
• Comparison of Stool DNA Isolation Methods
• Determination of DNA Molecular Weight
• Gut Microbiome Diversity: Comparison of Preservation Methods

Safety Data Sheets

• Dx27600 - Stool DNA Isolation Kit Dx - SDS

Flyers

• Stool DNA Isolation Kits

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Citations

Title	Journal	Authors
Changes on fecal microbiota in rats exposed to permethrin during postnatal development	Environmental Science and Pollution Research International (2016)	Nasuti C et al.
Independent Evaluation of CryoXtract Instruments’ CXT350	Biopreservation and Biobanking (2016)	Mathieson W et al.
Cryptosporidiosis outbreak in a child day-care center	Enfermedades Infecciosas y Microbiología Clínica (2015)	Pilar Goñi et al.
Detection of Campylobacter in human faecal samples in Fiji	Western Pacific Surveillance and Response Journal (2014)	Aruna Devi et al.
DNA-based diagnostic tools in veterinary parasitology	Veterinary Parasitology (2011)	Hunt PW, Lello J

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Related Services

• NGS (Next Generation Sequencing)

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Related Products

• Stool Nucleic Acid Collection and Preservation Devices Dx
• Stool Total RNA Purification Kit Dx

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