Norgen Stool DNA Isolation Kit Dx

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CE-IVDR 인증을 받은 진단용 대변 DNA 정제 키트로, 숙주 및 미생물 DNA를 동시에 빠르고 간편하게 추출합니다. PCR 저해물 제거로 고품질 DNA 확보, Norgen 보존 튜브와 완벽 호환, 다양한 분자진단 및 NGS 응용에 적합합니다.

브랜드: Norgen
카테고리: Genomic DNA Purification Systems and Kits
판매단위: pk

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Norgen · Norgen Stool DNA Isolation Kit Dx

Norgen Stool DNA Isolation Kit Dx

SKU: Dx27600
For the rapid and simple purification of bacterial and host DNA from stool and fecal samples for in vitro diagnostic use.

Features and Benefits

CE-IVDR marked in accordance with European Commission Regulation (EU) No. 2017/746
Ideal for use in in vitro diagnostic workflows
Simultaneous isolation of both host DNA and microbial DNA (universal protocol)
Eliminates PCR inhibitors including humic acids
Fully compatible with Norgen’s Stool Nucleic Acid Collection and Transport Tubes
High-quality DNA for sensitive downstream applications including PCR, qPCR, sequencing, and microarray

This kit provides a convenient and rapid method to isolate total DNA from fresh, frozen, and preserved stool samples, including those preserved using Norgen’s Stool Nucleic Acid Collection and Preservation Devices Dx (Cat. Dx45660).
The universal protocol allows simultaneous isolation of total genomic DNA from microorganisms and host cells.
All traces of humic acids and other inhibitors are removed using Bead Tubes and a combination of chemical and physical homogenization and lysis, without phenol-chloroform extraction.
A simple spin column procedure yields high molecular weight DNA (up to 50 kb+), suitable for PCR, qPCR, NGS, and microarray applications.

NOTE: This product is not available for sale in the United States.

Kit Specifications

Specification	Description
Maximum Stool Input	200 mg (fresh/frozen) or 400 μL (preserved)
Type of Stool Processed	Frozen, fresh, or preserved stool
Format	Spin Column
Maximum Column Binding Capacity	50 μg
Maximum Column Loading Volume	650 μL
Elution Volume	50 μL
Time to Complete 10 Purifications	30 minutes
Applications	PCR, qPCR, Southern Blot, Sequencing, Microarray Analysis

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature.
This kit is stable for 2 years after the date of shipment.

Component	Cat. Dx27600 (50 preps)
Lysis Solution	60 mL
Lysis Additive	6 mL
Binding Solution	7 mL
Wash Solution I	30 mL
Wash Solution II	22 mL
Elution Buffer	3 mL × 2
Bead Tube	50
Mini Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1

Supporting Data

Figure 1. Higher Yields of DNA than Competitor Z

Stool DNA was isolated from 200 mg of fresh stool samples using Norgen’s Stool DNA Isolation Kit Dx and Competitor Z’s Kit.
10 µL of DNA from the elution was run on 1X TAE 1.2% agarose gel.
Norgen’s kit isolated much higher yields of DNA.
Marker = Norgen’s HighRanger DNA Ladder.

Norgen	Competitor Z

Figure 2. Higher Quality DNA than Competitor Z

DNA quality measured by Nanodrop. Norgen’s Stool DNA Isolation Kit Dx isolated DNA of the highest quality.

Sample	A260/280 (OD)	A260/230 (OD)
Norgen	~2.0	~1.5
Competitor Z	~1.0	~0.3

Figure 3. High Quality DNA confirmed by Real-time PCR

Stool DNA was isolated from 200 mg of fresh stool samples using Norgen’s kit and Competitor Z’s kit.
DNA quality confirmed by real-time PCR detecting 16S rRNA.
Earlier Ct values with Norgen’s samples indicate higher quality DNA.

Amplification Graph Parameters

Y-axis: RFU (Relative Fluorescence Units) [0–2000]
X-axis: Cycles [0–40]

Figure 4. Taxonomic Classifications of 10 Fecal Microbiomes

A) Principal Coordinate Analysis (PCoA) showing taxonomic distribution up to class level.
B) Hierarchical clustering based on genus-level classifications.

Axis	Label
X-axis	Principal Coordinate 2
Y-axis	Principal Coordinate 1

Taxonomic Levels: Kingdom, Phylum, Class, Order, Family, Genus, Species

Documentation

Protocols

(Dx27600) Stool DNA Isolation Kit Dx - Protocol (50 Preps)

Application Notes

Safety Data Sheets

Dx27600 - Stool DNA Isolation Kit Dx - SDS

Flyers

Stool DNA Isolation Kits

Citations

Title	Journal	Authors
Changes on fecal microbiota in rats exposed to permethrin during postnatal development	Environmental Science and Pollution Research International (2016)	Nasuti C et al.
Independent Evaluation of CryoXtract Instruments’ CXT350	Biopreservation and Biobanking (2016)	Mathieson W et al.
Cryptosporidiosis outbreak in a child day-care center	Enfermedades Infecciosas y Microbiología Clínica (2015)	Pilar Goñi et al.
Detection of Campylobacter in human faecal samples in Fiji	Western Pacific Surveillance and Response Journal (2014)	Aruna Devi et al.
DNA-based diagnostic tools in veterinary parasitology	Veterinary Parasitology (2011)	Hunt PW, Lello J

NGS (Next Generation Sequencing)

제품 이미지

Figure 1. Higher Yields of DNA than Competitor Z
Figure 2. Higher Quality DNA than Competitor Z
Figure 3. High Quality DNA confirmed by Real-time PCR
Figure 4. Taxonomic Classifications of 10 Fecal Microbiomes