Thermo Fisher Scientific Phosphotyrosine Monoclonal Antibody (pY20), FITC
FITC 결합된 pY20 단클론 항체로, 인산화 티로신 잔기를 특이적으로 인식합니다. Western blot, IHC, ELISA 등 다양한 응용에 적합하며, Protein A/G 정제된 고순도 항체입니다. 세린/트레오닌 인산화 잔기에는 반응하지 않습니다.
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Thermo Fisher Scientific Phosphotyrosine Monoclonal Antibody (pY20), FITC
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | 5 publications |
| Immunohistochemistry (IHC) | 5–10 µg/mL | 1 publication |
| Immunocytochemistry (ICC/IF) | – | 1 publication |
| ELISA | Assay-dependent | – |
| Immunoprecipitation (IP) | Assay-dependent | 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Published Species | Chemical |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | pY20 |
| Immunogen | Phosphotyrosine coupled to carrier protein |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.5, with 50% glycerol |
| Contains | 3 mM sodium azide |
| Storage Conditions | –20°C, avoid freeze/thaw cycles, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1086658 |
Additional Formats
- Unconjugated (Cat. AHO0681)
- Biotin (Cat. MA1-12439)
- HRP (Cat. MA1-12445)
- Custom conjugation available upon request
Product Specific Information
This antibody does not react with phosphorylated threonine or serine residues.
Target Information
Tyrosine phosphorylation plays a critical role in signal transduction from transmembrane receptors and in oncogene tyrosine kinase transformation. Antibodies that specifically recognize phosphorylated tyrosine residues are invaluable for studying tyrosine-phosphorylated proteins and related biochemical pathways. The FITC-conjugated clone pY20 anti-phosphotyrosine antibody enables direct detection of P-Tyr proteins in immunohistochemical and immunocytochemical assays without the need for secondary antibodies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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