Thermo Fisher Scientific Phosphotyrosine Monoclonal Antibody (pY20), Biotin
Biotin으로 결합된 pY20 monoclonal antibody로, phosphotyrosine 잔기를 특이적으로 인식합니다. Western blot, IHC, ICC, ELISA, IP 등 다양한 응용에 적합하며, 고순도 Protein A/G 정제 제품입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:5,000 | View 4 publications |
| Immunohistochemistry (IHC) | 1:500 | View 2 publications |
| Immunocytochemistry (ICC/IF) | 1:500 | View 1 publication |
| ELISA | 1:5,000 | - |
| Immunoprecipitation (IP) | Assay-dependent | View 2 publications |
| Miscellaneous (PubMed) | - | View 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Published Species | Chemical |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | pY20 |
| Immunogen | Phosphotyrosine coupled to carrier protein |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 65% glycerol |
| Contains | 0.08% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1086656 |
Available Formats
Target Information
Tyrosine phosphorylation plays a critical role in mitogenic signal transduction from transmembrane receptors and in transformation by oncogene tyrosine kinases. Phosphorylation of specific tyrosine residues is a key mechanism in normal mitogenesis, cell cycle progression, and oncogenic transformation, and continues to be studied in differentiation and gap junction communication.
Antibodies that specifically recognize phosphorylated tyrosine residues, such as clone pY20, are invaluable tools for studying tyrosine-phosphorylated proteins and related biochemical pathways. The fluorescein (FITC) conjugate of clone pY20 is particularly useful for detecting phosphotyrosine-containing proteins in immunohistochemical and immunocytochemical protocols where secondary antibody use may complicate detection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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