
Thermo Fisher Scientific Calpain S1/S2 Monoclonal Antibody (28F3)
Calpain S1/S2 단백질의 28~30kDa 소단위를 특이적으로 인식하는 단클론 항체. Western blot, ELISA, IP에 사용 가능. 인간 및 소 반응성. Protein A/G 정제, 1mg/mL 농도, PBS/glycerol buffer에 보관. 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–1 µg/mL |
| ELISA | 0.5–1.0 µg/mL |
| Immunoprecipitation (IP) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 28F3 |
| Immunogen | Human Calpain protein purified from placenta |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.5, with 50% glycerol |
| Contains | 3 mM sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_1070905 |
Product Specific Information
This antibody specifically recognizes the 28 to 30 kDa small subunit of Calpain 1 and Calpain 2 (μ-calpain and m-calpain). It recognizes both native and denatured protein in ELISA, Western Blot, and Immunoprecipitation assays.
Target Information
The calpains are calcium-dependent cysteine proteases widely expressed in mammalian systems. Both m-calpain (calpain II) and μ-calpain (calpain I) consist of an 80 kDa catalytic subunit and a 30 kDa regulatory subunit. The 30 kDa subunit is common to both enzymes, while the catalytic subunits differ in their calcium requirements: m-calpain requires millimolar (mM) levels of Ca²⁺, whereas μ-calpain is active at micromolar (µM) concentrations. In addition to these ubiquitous forms, tissue-specific calpains have been identified. Calpains play multiple physiological roles, and research on their functions continues to evolve.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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