Thermo Fisher Scientific UBC9 Recombinant Rabbit Monoclonal Antibody (SC0534)
UBC9 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC/IF에 사용 가능. HEK293 세포 발현, 높은 특이성과 일관성 제공. 단백질 A 정제, 1 mg/mL 농도, 4°C 또는 -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SC0534 |
| Immunogen | Synthetic peptide within Human UBC9 aa 15–60 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809487 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and expressed in these systems. Individual clones are screened to select the best candidates for production.
Advantages include:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broad immunoreactivity due to the rabbit immune repertoire
Target Information
The modification of proteins with ubiquitin is an essential cellular mechanism for degrading abnormal or short-lived proteins.
Ubiquitination involves three enzyme classes:
- Ubiquitin-activating enzymes (E1s)
- Ubiquitin-conjugating enzymes (E2s)
- Ubiquitin-protein ligases (E3s)
This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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