Thermo Fisher Scientific IL-17A Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.25–1.0 µg/mL
• Publications: 1 publication available

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
AAIIPQSSAC PNTEAKDFLQ NVKVNLKVFN SLGAKVSSRR PSDYLNRSTS PWTLHRNEDP DRYPSVIWEA QCRHQRCVNA EGKLDHHMNS VLIQQEILVL KREPESCPFT FRVEKMLVGV GCTCVASIVR QAA

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-17A. Anti-Murine IL-17A-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect mIL-17A by sandwich ELISA (100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine IL-17A (500-P265) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant mIL-17A.

Western Blot:
To detect mIL-17A by Western Blot analysis, use at 0.1–0.2 µg/mL. With compatible secondary reagents, detection limit for Recombinant mIL-17A is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P265BT-1MG is provided as 2 × 500 µg.

Target Information

Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection.
IL-17A is a 32 kDa disulfide-linked homodimer (136 amino acids) belonging to the IL-17 family (IL-17A–F) and signals through IL-17R/CDw217.
It enhances ICAM-1 expression and induces secretion of IL-6, IL-8, G-CSF, and PGE2 in epithelial, endothelial, and fibroblastic cells.
IL-17A regulates NF-κB and MAPK pathways, stimulates IL-6 and COX-2 expression, and increases nitric oxide production.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.