Thermo Fisher Scientific PEDF Polyclonal Antibody, Biotin, PeproTech
Biotin으로 표지된 Rabbit Polyclonal Anti-Human PEDF 항체로, Western blot(0.1–0.2 µg/mL) 및 ELISA(0.25–1.0 µg/mL)에 적합합니다. Affinity chromatography로 정제되었으며, Lyophilized 형태로 제공됩니다. 신경보호 및 항혈관신생 연구에 유용합니다.
- 카탈로그번호
- 500-P263BT-xxxx (2개 옵션)
- 판매단위
- pk
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific PEDF Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.25–1.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human PEDF |
| Conjugate | Biotin |
| Form | Lyophilized |
| Purification | Affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2942219 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
MQNPASPPEE GSPDPDSTGA LVEEEDPFFK VPVNKLAAAV SNFGYDLYRV RSSMSPTTNV LLSPLSVATA LSALSLGAEQ RTESIIHRAL YYDLISSPDI HGTYKELLDT VTAPQKNLKS ASRIVFEKKL RIKSSFVAPL EKSYGTRPRV LTGNPRLDLQ EINNWVQAQM KGKLARSTKE IPDEISILLL GVAHFKGQWV TKFDSRKTSL EDFYLDEERT VRVPMMSDPK AVLRYGLDSD LSCKIAQLPL TGSMSIIFFL PLKVTQNLTL IEESLTSEFI HDIDRELKTV QAVLTVPKLK LSYEGEVTKS LQEMKLQSLF DSPDFSKITG KPIKLTQVEH RAGFEWNEDG AGTTPSPGLQ PAHLTFPLDY HLNQPFIFVL RDTDTGALLF IGKILDPRGP
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human PEDF. Anti-Human PEDF-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect Human PEDF by sandwich ELISA (100 µL/well), use at 0.25–1.0 µg/mL. When paired with PeproTech Polyclonal Anti-Human PEDF (500-P263) as a capture antibody, detection sensitivity is 2000–4000 pg/mL of Recombinant Human PEDF.
Western Blot:
For detection of Human PEDF by Western blot, use at 0.1–0.2 µg/mL. Detection limit: 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Package:
500-P263BT-1MG is supplied as 2 × 500 µg.
Target Information
PEDF (Pigment Epithelium-Derived Factor) is a noninhibitory serpin with neurotrophic, anti-angiogenic, and anti-tumorigenic properties. It is a 50 kDa glycoprotein expressed in various tissues. PEDF induces apoptosis in proliferating endothelial cells and inhibits angiogenic factors such as VEGF and FGF-2. Its neuroprotective effects are mediated through suppression of neuronal apoptosis caused by oxidative stress or neurotoxins.
PEDF-R, a lipase-linked cell membrane receptor, binds PEDF with high affinity and plays a key role in its signaling. PEDF is considered a potential therapeutic target for vasculature-related neurodegenerative diseases such as age-related macular degeneration (AMD) and proliferative diabetic retinopathy (PDR), as well as angiogenesis-related cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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