Thermo Fisher Scientific PEDF Polyclonal Antibody, PeproTech
인간 PEDF 단백질을 인식하는 토끼 폴리클로날 항체로, Western blot 및 ELISA에 적합합니다. 고순도 재조합 단백질로 면역화되어 친화 크로마토그래피로 정제되었습니다. 신경보호 및 항혈관신생 연구에 유용하며, -20°C에서 보관합니다.
- 카탈로그번호
- 500-P263-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific PEDF Polyclonal Antibody, PeproTech
Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.25–1.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli derived Recombinant Human PEDF |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2942215 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
MQNPASPPEE GSPDPDSTGA LVEEEDPFFK VPVNKLAAAV SNFGYDLYRV RSSMSPTTNV LLSPLSVATA LSALSLGAEQ RTESIIHRAL YYDLISSPDI HGTYKELLDT VTAPQKNLKS ASRIVFEKKL RIKSSFVAPL EKSYGTRPRV LTGNPRLDLQ EINNWVQAQM KGKLARSTKE IPDEISILLL GVAHFKGQWV TKFDSRKTSL EDFYLDEERT VRVPMMSDPK AVLRYGLDSD LSCKIAQLPL TGSMSIIFFL PLKVTQNLTL IEESLTSEFI HDIDRELKTV QAVLTVPKLK LSYEGEVTKS LQEMKLQSLF DSPDFSKITG KPIKLTQVEH RAGFEWNEDG AGTTPSPGLQ PAHLTFPLDY HLNQPFIFVL RDTDTGALLF IGKILDPRGP
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human PEDF. Anti-Human PEDF-specific antibody was purified by affinity chromatography employing an immobilized Human PEDF matrix.
Sandwich ELISA:
To detect Human PEDF by sandwich ELISA (using 100 µL/well), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human PEDF (500-P263) as a capture antibody, allows the detection of at least 2000–4000 pg/mL of Recombinant Human PEDF.
Western Blot:
To detect Human PEDF by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible development reagents, the detection limit for Recombinant Human PEDF is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
PEDF (Pigment Epithelium-Derived Factor) is a noninhibitory serpin with neurotrophic, anti-angiogenic, and anti-tumorigenic properties. It is a 50 kDa glycoprotein produced and secreted in many tissues throughout the body.
A major component of PEDF’s anti-angiogenic action is the induction of apoptosis in proliferating endothelial cells. PEDF also inhibits angiogenic factors such as VEGF and FGF-2.
Its neuroprotective effects are achieved through suppression of neuronal apoptosis induced by oxidative or excitotoxic stress. PEDF binds to a specific receptor (PEDF-R), a lipase-linked cell membrane receptor, which mediates its signaling functions.
Due to its multifunctional nature, PEDF is considered a potential therapeutic target in vasculature-related neurodegenerative diseases (e.g., AMD, PDR) and various cancers involving angiogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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