Thermo Fisher Scientific IL-17A Polyclonal Antibody, PeproTech
마우스 IL-17A를 인식하는 토끼 폴리클로날 항체로, Western blot과 ELISA에 최적화되어 있습니다. 항원 친화 크로마토그래피로 정제되었으며, 고순도 재조합 단백질을 면역원으로 사용합니다. 연구용으로 염증 반응 및 사이토카인 신호 분석에 적합합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.5–2.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Murine IL-17A |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930146 |
Product Specific Information
AA Sequence of recombinant protein:
AAIIPQSSAC PNTEAKDFLQ NVKVNLKVFN SLGAKVSSRR PSDYLNRSTS PWTLHRNEDP DRYPSVIWEA QCRHQRCVNA EGKLDHHMNS VLIQQEILVL KREPESCPFT FRVEKMLVGV GCTCVASIVR QAA
Preparation:
Produced from sera of rabbits immunized with highly pure recombinant murine IL-17A. Anti-murine IL-17A-specific antibody was purified by affinity chromatography employing an immobilized murine IL-17A matrix.
Sandwich ELISA:
To detect murine IL-17A by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine IL-17A (500-P265Bt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of recombinant murine IL-17A.
Western Blot:
To detect murine IL-17A by Western blot analysis, use at 0.1–0.2 µg/mL. In conjunction with compatible secondary reagents, the detection limit for recombinant murine IL-17A is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Target Information
Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection.
IL-17A is a 32 kDa disulfide-linked homodimer consisting of 136 amino acids, part of the IL-17 family (IL-17A–17F), signaling through IL-17R (CDw217).
Human IL-17A stimulates epithelial, endothelial, and fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. It enhances ICAM-1 expression and supports proliferation of CD34+ hematopoietic progenitors.
Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating receptor cross-reactivity. IL-17A regulates NF-κB and MAPK activities, stimulates IL-6 and COX-2 expression, and enhances nitric oxide production.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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