Thermo Fisher Scientific IL-17A Polyclonal Antibody, Biotin, PeproTech
Biotin으로 표지된 Rabbit Polyclonal Anti-Human IL-17A 항체로, Western Blot, IHC, ELISA 등 다양한 응용에 적합. 고순도 재조합 IL-17A 항원으로 면역화된 후 항원 친화 크로마토그래피로 정제. 염증성 사이토카인 연구에 유용하며 -20°C에서 안정적으로 보관 가능.
- 카탈로그번호
- 500-P07BT-xxxx (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL-17A Polyclonal Antibody, Biotin, PeproTech
Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| Immunohistochemistry (IHC) | - | View 1 publication |
| ELISA | 0.25–1.0 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Mouse |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human IL-17A |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929175 |
Product Specific Information
AA Sequence of Recombinant Protein:
MIVKAGITIP RNPGCPNSED KNFPRTVMVN LNIHNRNTNT NPKRSSDYYN RSTSPWNLHR NEDPERYPSV IWEAKCRHLG CINADGNVDY HMNSVPIQQE ILVLRREPPH CPNSFRLEKI LVSVGCTCVT PIVHHVA.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IL-17A. Anti-Human IL-17A-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect Human IL-17A by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-17A (500-P07) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human IL-17A.
Western Blot:
To detect hIL-17A by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL.
Used with compatible secondary reagents, the detection limit for Recombinant hIL-17A is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Packaging:
500-P07BT-1MG is provided as 2 × 500 µg.
Target Information
Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection.
IL-17A is a 32 kDa disulfide-linked homodimer consisting of 136 amino acids and is part of the IL-17 family (IL-17A–17F). It signals through the IL-17 receptor (IL-17R/CDw217).
High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of ICAM-1 in human fibroblasts and stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2.
In the presence of IL-17A, fibroblasts can sustain proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils.
Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating cross-species receptor recognition.
IL-17A regulates NF-kappa B and MAPK activities, stimulates IL-6 and COX-2 expression, and enhances nitric oxide production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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