Thermo Fisher Scientific IL-17A Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific IL-17A Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MIVKAGITIP RNPGCPNSED KNFPRTVMVN LNIHNRNTNT NPKRSSDYYN RSTSPWNLHR NEDPERYPSV IWEAKCRHLG CINADGNVDY HMNSVPIQQE ILVLRREPPH CPNSFRLEKI LVSVGCTCVT PIVHHVA.

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human IL-17A. Anti-Human IL-17A-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human IL-17A by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-17A (500-P07G) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human IL-17A.

Western Blot:
To detect hIL-17A by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant hIL-17A is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Package Information:
500-P07GBT-1MG will be provided as 2 × 500 µg.

Target Information

Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection.
IL-17A is a 32 kDa disulfide-linked homodimer consisting of 136 amino acids, belonging to the IL-17A–17F family, and signals through the IL-17 receptor (IL-17R/CDw217).
Human IL-17A stimulates epithelial, endothelial, and fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2, and supports proliferation and maturation of CD34+ hematopoietic progenitors into neutrophils.
It regulates NF-kappa B and MAP kinase activities, enhances IL-6 and COX-2 expression, and increases nitric oxide (NO) production.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.