Thermo Fisher Scientific TNFR2 (soluble) Polyclonal Antibody, Biotin, PeproTech
Human TNFR2 단백질을 인식하는 Biotin 결합 토끼 폴리클로날 항체. Western blot 및 ELISA에 적합하며, 높은 특이성과 민감도로 sTNF Receptor II 검출 가능. 항원 친화 크로마토그래피로 정제되어 연구용으로 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.25–1.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived, 18.9 kDa Recombinant Human TNF Receptor Type II |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929604 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
MAPEPGSTCR LREYYDQTAQ MCCSKCSPGQ HAKVFCTKTS DTVCDSCEDS TYTQLWNWVP ECLSCGSRCS SDQVETQACT REQNRICTCR PGWYCALSKQ EGCRLCAPLR KCRPGFGVAR PGTETSDVVC KPCAPGTFSN TTSSTDICRP HQICNVVAIP GNASMDAVCT STSP.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human sTNF Receptor Type II. Anti-Human sTNF Receptor Type II-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect hsTNF Receptor II by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sTNF Receptor II (500-P168) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant hsTNF Receptor II.
Western Blot:
To detect hsTNF Receptor II by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant hsTNF Receptor II is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Package Information:
500-P168BT-1MG is provided as 2 × 500 µg.
Target Information
TNFR2 (tumor necrosis factor receptor superfamily member 1B) is a receptor with high affinity for TNFSF2/TNF-alpha and lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. TNFR2 mediates most of the metabolic effects of TNF-alpha.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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