Thermo Fisher Scientific MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), NovaFluor Blue 585, eBioscience

Thermo Fisher Scientific MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), NovaFluor Blue 585

Applications

• Flow Cytometry

Tested Dilution

• 1.0 µg/test

Product Specifications

Alternative Formats

• Unconjugated
• APC
• Biotin
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 710
• NovaFluor Red 755
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Request custom conjugation

Product Specific Information

The 28-14-8 monoclonal antibody reacts with mouse MHC class I, H-2D^b, and cross-reacts with H-2L^d, H-2D^q and/or H-2L^q. Binding to the alpha 3 domain of H-2L^d is not dependent on beta2-microglobulin.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Validated for flow cytometric analysis of mouse splenocytes at ≤1 µg/test. A test is defined as staining a cell sample in a final volume of 100 µL. Recommended titration for optimal performance.

Compatibility Notes:
NovaFluor dyes are not compatible with DNA intercalating viability dyes such as propidium iodide, 7-AAD, or DAPI. Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment.

Buffer Information:
This conjugate ships with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01), optimized for NovaFluor conjugates. Add 5 µL per sample to antibody cocktails/master mixes prior to combining with cells.

Technology:
NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures designed to minimize spillover and spread effects.

Optical Properties:

• Excitation: 494 nm
• Emission: 585 nm
• Laser: 488 nm (Blue)

Target Information

MHC class I alloantigen involved in antigen presentation to CD3/TCR and CD8-expressing T cells.
HLA and MHC antibodies are essential for immunopeptidomics applications, enabling identification and characterization of neoantigens through LC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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