Thermo Fisher Scientific MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), NovaFluor Blue 510, eBioscience

Thermo Fisher Scientific MHC Class I (H-2Db) Monoclonal Antibody (28-14-8), NovaFluor Blue 510

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1.0 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• Biotin
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 585
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 710
• NovaFluor Red 755
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Request custom conjugation

Product Specific Information

The 28-14-8 monoclonal antibody reacts with the mouse MHC class I, H-2D^b, and cross-reacts with H-2L^d, H-2D^q and/or H-2L^q.
Binding of 28-14-8 to the alpha 3 domain of H-2L^d is not dependent on beta2-microglobulin.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Tested with mouse splenocytes at ≤1 µg/test (100 µL final volume). Recommended to titrate for optimal performance.

Note:
NovaFluor dyes are not compatible with DNA intercalating viability dyes such as propidium iodide, 7-AAD, and DAPI.
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.

This conjugate ships with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01), which provides improved formulation over CellBlox.
Use 5 µL per sample for optimal staining. For single-color controls, use 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates are based on Phiton™ technology, utilizing novel nucleic acid dye structures for engineered fluorescent signatures optimized for spillover and spread impacts.

Excitation: 496 nm
Emission: 511 nm
Laser: 488 nm (Blue)

Target Information

This protein is an MHC class I alloantigen involved in antigen presentation to CD3/TCR and CD8 T cells.
HLA and MHC antibodies play a key role in Immunopeptidomics, aiding identification and characterization of neoantigens via LC-MS/MS.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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