Thermo Fisher Scientific Ubiquitin Monoclonal Antibody (6C11-B3), APC
APC 형광 표지된 Ubiquitin 단일클론 항체로 다양한 종에서 반응하며 WB, ICC/IF, ELISA에 사용 가능. 단백질 분해 관련 연구에 적합하며 고순도 Protein G 정제 제품. 4°C 보관, 무보존제 포맷.
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Thermo Fisher Scientific Ubiquitin Monoclonal Antibody (6C11-B3), APC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ELISA | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | All, Bovine, Human, Mouse, Rat |
| Host/Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6C11-B3 |
| Immunogen | Native bovine ubiquitin conjugated to KLH |
| Conjugate | APC |
| Excitation/Emission Max | 651/660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931684 |
Additional Formats
Product Specific Information
1 µg/mL of MA5-45230 was sufficient for detection of ubiquitin in 10 µg of HeLa lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 10 kDa.
Target Information
Ubiquitin is a highly conserved protein involved in targeting cellular proteins for degradation by the 26S proteasome. It is synthesized as a precursor protein consisting of polyubiquitin chains or a single ubiquitin fused to an unrelated protein. The gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. Post-translational processing generates free ubiquitin monomer and ribosomal protein S27a, a component of the 40S ribosomal subunit. Ribosomal protein S27a contains C4-type zinc finger domains and is localized in the cytoplasm. Pseudogenes derived from this gene are present in the genome. Ribosomal proteins L40 and S30 are also synthesized as fusion proteins with ubiquitin or ubiquitin-like proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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