Thermo Fisher Scientific HDEL Monoclonal Antibody (2E7), APC

Applications and Tested Dilutions

Product Specifications

Available Formats

• Unconjugated (MA5-27549)
• FITC (MA5-45241)
• PE (MA5-45243)
• PerCP (MA5-45242)
• Custom conjugation available upon request

Product Specific Information

1 µg/mL of MA5-45240 was sufficient for detection of HDEL-containing proteins in 10 µg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 78 kDa.

Target Information

HSP70 family comprises four highly conserved proteins—HSP70, HSC70, GRP75, and GRP78—serving as molecular chaperones. They facilitate assembly of multi-protein complexes, assist in translocation across membranes, and aid in proper folding of nascent polypeptides.
GRP78, localized in the endoplasmic reticulum (ER), assists in folding and translocation of secretory proteins. Sorting of these proteins depends on a C-terminal tetrapeptide signal, typically KDEL in animal cells and HDEL in S. cerevisiae.
The 2E7 clone recognizes the C-terminal peptide HDEL, a common ER retention signal found in yeast, plants, nematodes, and other ER proteins. It specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum, and wheat.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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