Thermo Fisher Scientific HDEL Monoclonal Antibody (2E7), APC
HDEL 서열을 인식하는 마우스 단클론 항체로, APC 형광으로 표지되어 있습니다. 효모, 식물 등 다양한 종의 ER 단백질 검출에 적합하며, WB 및 ICC/IF에 사용 가능합니다. 단백질 G로 정제된 액상 형태로 4°C에서 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Fruit fly, Plant, Yeast |
| Host/Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2E7 |
| Immunogen | Synthetic HDEL peptide corresponding to the C-terminus of yeast Bip |
| Conjugate | APC |
| Excitation/Emission Max | 651/660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931694 |
Available Formats
- Unconjugated (MA5-27549)
- FITC (MA5-45241)
- PE (MA5-45243)
- PerCP (MA5-45242)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45240 was sufficient for detection of HDEL-containing proteins in 10 µg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 78 kDa.
Target Information
HSP70 family comprises four highly conserved proteins—HSP70, HSC70, GRP75, and GRP78—serving as molecular chaperones. They facilitate assembly of multi-protein complexes, assist in translocation across membranes, and aid in proper folding of nascent polypeptides.
GRP78, localized in the endoplasmic reticulum (ER), assists in folding and translocation of secretory proteins. Sorting of these proteins depends on a C-terminal tetrapeptide signal, typically KDEL in animal cells and HDEL in S. cerevisiae.
The 2E7 clone recognizes the C-terminal peptide HDEL, a common ER retention signal found in yeast, plants, nematodes, and other ER proteins. It specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum, and wheat.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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